What Can We Learn from a Pig Model of FAP?

A pig model of intestinal adenoma development, described in the November issue of Gastroenterology, will improve our understanding of colorectal cancer development and could be used to evaluate new therapeutics.

Familial adenomatous polyposis (FAP) is an inherited disease; patients develop dysplasias in the colon and rectum that develop to adenomatous polyps and adenocarcinoma. FAP is caused by germline mutations in adenomatous polyposis coli (APC), which encodes a tumor suppressor—somatic mutations in APC are found in early-stage sporadic colorectal tumors.

Although there are several mouse models of APC, they don’t fully resemble the human disease.

Tatiana Flisikowska et al. show that in pigs, unlike mice, a single heterozygous germline mutation in APC is sufficient to initiate the sequence that leads to adenomas in the large intestine, and replicate early-stage human FAP.

Using cloned pigs, Flisikowska et al. inserted translational stop signals in APC at codons 1061 and 1311—orthologous to common germline mutations (APC1061 and APC1309) that cause human FAP. The mutation at human codon 1309 is associated with a particularly severe phenotype, with early onset and prolific polyposis; a mutation at codon 1061 causes a less-severe form of polyposis.

After 1 year, wild-type pigs had normal gastrointestinal tracts. The intestines of the APC1061/+ pig showed no evidence of polyposis. However, colon and rectal tissues from each APC1311/+ pig had more than 100 macroscopically visible lesions, including more than 60 sessile polyps. Polyps ranged from barely visible mucosal nodules of 1–2 mm to flat polyps up to 1 cm. They were scattered along the entire large bowel, with most in the proximal colon (see below figure).

Macroscopically visible intestinal lesions in APC1311/+ pigs. Red circles indicate positions of polyps with dysplasia (adenomas); yellow circles indicate hyperplastic polyps without dysplasia.

As in young patients with FAP, no gastric polyps or duodenal adenomas were observed in the pigs. Colonic adenomas develop during childhood, but gastric polyps and duodenal adenomas occur later, when patients reach adulthood.

Importantly for research purposes, the pigs with FAP could be evaluated using human-sized equipment, such as high-resolution magnification chromoendoscopic imaging of the small and large intestines.

Pathology evaluation of the pigs’ lesions showed serrated morphology without dysplasia, which are common in humans but not in mice with Apc mutations. These types of lesions increase risk of colorectal cancer in humans.

Some larger adenomas showed focal features of more advanced tumors, and were classified as adenomas with focal high-grade intraepithelial neoplasia. The pigs’ tumors appeared identical to human colonic adenomas with respect to surface involvement, with dysplastic cells on the superficial mucosal surface—this is in contrast to the adenomas that form in Apc mutant mice, which have a surface layer of nondysplastic epithelium.

In an editorial that accompanies the article, Joanna Groden and Randall Burt say that the new model created by Flisikowska et al. will permit the study of allelic variation at the APC locus and the polyposis phenotype.

Animal models of intestinal tumor formation have been important in identifying mechanisms of cancer pathogenesis, but mouse models are limited because most adenomas form in the small intestine, as well as their histopathology features and short lifespan, which prevents studies of cancer progression and therapies.

How do mutations in APC lead to adenoma and adenocarcinoma? APC is part of a complex that regulates the phosphorylation-dependent degradation of β-catenin and Wnt signaling. Cancer-associated mutations in APC most commonly insert stop codons onto the open reading frame, to truncate APC. It can therefore no longer bind or contribute to degradation of β-catenin, leading to constitutive stabilization of β-catenin and the expansion of the stem cell compartment in the intestinal crypts.

Flisikowska et al. assessed the wild-type APC allele in 5 porcine adenomas (0.4–1 cm; 3 with low-grade and 2 with low- and focal high-grade dysplasia). The wild-type APC allele was lost in each case. Aberrant crypt foci and colorectal tumors had high levels of nuclear and cytoplasmic β-catenin staining outside the proliferative zone, indicating that tumor initiation and progression occurs via Wnt pathway activation. Consistent with human FAP, there was no increase in epithelial proliferation of histologically normal intestinal mucosa.

Groden and Burt say that an animal model that more closely resembles human disease will improve our understanding of the relationships between APC genotype and phenotypes, and led to identification of other genes that affect disease development and therapeutic targets.

Read the article online.
Flisikowska T, Merkl C, Landmann M, et al. A porcine model of familial adenomatous polyposis.Gastroenterology 2012;143:1173−1175.e7.

Read the accompanying editorial.
Groden J, Burt R. Genotypes and phenotypes: Animal models of familial adenomatous polyposis coli. Gastroenterology 2012;143:1133−1135.

About these ads

About Kristine Novak, PhD, Science Editor

Dr. Kristine Novak is the science editor for Gastroenterology and Clinical Gastroenterology and Hepatology, both published by the American Gastroenterological Association. She has worked as an editor at biomedical research journals and as a science writer for more than 12 years, covering advances in gastroenterology, hepatology, cancer, immunology, biotechnology, molecular genetics, and clinical trials. She has a PhD in cell biology and an interest in all areas of medical research.
This entry was posted in Cancer, GI Tract, Technology and tagged , , , , , , , , , . Bookmark the permalink.

3 Responses to What Can We Learn from a Pig Model of FAP?

  1. Actually I had heard the name of FAP many times but was not very clear as to what the disease actually is. You have explained it in the most elaborative manner. Thank you so much.

  2. Hello I am so thrilled I found your weblog, I really found you by error, while I was searching on Google for
    something else, Nonetheless I am here now and would just like
    to say thank you for a marvelous post and a all
    round interesting blog (I also love the theme/design),
    I don’t have time to read through it all at the moment but I
    have book-marked it and also added in your RSS feeds, so when I have time I will be back to read a great deal more, Please
    do keep up the fantastic jo.

  3. Ellie Beirne says:

    Have you ever thought about writing an ebook or guest authoring on other websites? I have a blog based upon on the same topics you discuss and would really like to have you share some stories/information. I know my viewers would value your work. If you are even remotely interested, feel free to send me an email. Ajax Roofing Contractors, 15-75 Bayly St. W., #323, Ajax, Ontario, L1S 7L7, Canada, 289-275-1500

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s